Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
September 5, 2026BMC Cardiovascular DisordersOpen Access

Downregulated serum miR-744-5p identifies ACS with a diagnostic AUC of ~0.91.

View Full Paper
Ask AI
Bookmark
Share

Population

159 participants, comprising 86 patients diagnosed with acute coronary syndrome and 73 healthy controls. ACS…

Design

Case-control

Key result

Serum miR-744-5p was significantly downregulated in patients with acute coronary syndrome, demonstrating high diagnostic efficacy with an AUC of 0.9103.

Authors

WWWenhua WangRLRongwen LinCZChangpeng Zuo

Discussion

Loading...

Member takes

Overview

Supports miR-744-5p as potential ACS biomarker; extends prior observations but leaves open clinical adoption pending prospective validation.

Key Points

  • To evaluate the diagnostic accuracy of serum miR-744-5p in patients with acute coronary syndrome and clarify its molecular role in endothelial injury and inflammation via filamin A regulation.
  • Quantified serum miR-744-5p expression using RT-qPCR in 86 patients with acute coronary syndrome and 73 healthy controls, evaluating diagnostic efficacy by ROC curve analysis.
  • Assessed cell proliferation, myocardial and endothelial injury markers (H-FABP, vWF), and pro-inflammatory factors in hypoxia/reoxygenation-stimulated human aortic endothelial cells.
  • Verified direct binding between miR-744-5p and filamin A (FLNA) using dual-luciferase reporter and RNA immunoprecipitation assays.
  • Serum miR-744-5p was significantly downregulated in acute coronary syndrome patients, demonstrating strong diagnostic performance with an AUC of 0.9103.
  • FLNA was significantly upregulated in acute coronary syndrome models and was confirmed as a direct downstream target of miR-744-5p.
  • Overexpression of miR-744-5p downregulated FLNA, suppressed pro-inflammatory cytokine secretion, decreased H-FABP and vWF release, and promoted endothelial cell proliferation.

Study Design

Type

Case-Control (n=159)

Multicenter

No

Structured PICO

P
Population
159 participants, comprising 86 patients with acute coronary syndrome and 73 healthy controls, were evaluated to determine the diagnostic efficacy of serum miR-744-5p.
O
Outcome
Diagnostic efficacy of serum miR-744-5p for acute coronary syndrome (measured by Area Under the Receiver Operating Characteristic Curve)surrogate

Main Result

Effect estimate: AUC 0.9103 (95% CI 0.8579-0.9627)

p-value: p=<0.0001

Serum miR-744-5p is significantly downregulated in acute coronary syndrome, demonstrating high diagnostic potential while mechanistically attenuating endothelial injury and inflammation by targeting FLNA.

Limitations

  • Specific downstream signaling pathways of FLNA remain to be fully elucidated.
  • Function of miR-744-5p within cardiomyocytes and its direct regulatory effect on cardiac function need further exploration.
  • Relatively small sample size prevents subgroup analysis of miR-744-5p expression across different ACS subtypes.
  • Findings are mainly derived from in vitro experiments and overexpression assays, requiring further in vivo validation.
  • The endogenous reference gene adopted for miRNA normalization exhibits lower stability than alternative endogenous references.

Cite This Study

Wang et al. (2026) conducted a case-control in Acute coronary syndrome (n=159). Serum miR-744-5p vs. Healthy controls was evaluated on Diagnostic performance for acute coronary syndrome (AUC 0.9103, 95% CI 0.8579-0.9627, p=<0.0001). Serum miR-744-5p was significantly downregulated in patients with acute coronary syndrome, demonstrating high diagnostic efficacy with an AUC of 0.9103.

synapsesocial.com/papers/6a9bd48c6b95aff0620ec388https://doi.org/10.1186/s12872-026-06412-5
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Dysregulation of Serum miR-212-3p Serves as a Biomarker to Predict Disease Onset and Short-Term Prognosis in Acute Coronary Syndrome Patients2025 · 1 citations
  2. 2miR-146b-5p serves as a novel biomarker for acute myocardial infarction and is involved in regulating oxidized low-density lipoprotein-induced endothelial injury through targeting PRKAR2A2026
  3. 3The Potential of Downregulated Serum miR-1282 to Predict Disease Onset and Short-Term Prognosis in Acute Coronary Syndrome Patients2025
  4. 4miR-3150b-3p Promotes Vascular Smooth Muscle Cell Injury by Targeting ARL5B2025 · 1 citations
  5. 5Suppression of circ_0091761 ameliorates acute myocardial infarction-induced endothelial injury through regulation of miR-12782025 · 3 citations