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October 15, 2025The EMBO JournalOpen Access

Single-molecule imaging of transcription dynamics, RNA localization and fate in human T cells

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Authors

MLMaria Valeria LattanzioNŠNikolina ŠoštarićNKNandhini Kanagasabesan

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Overview

Single-molecule FISH uncovers mRNA expression and post-transcriptional regulation in T cells, suggesting distinct cytokine pathways.

Key Points

  • T-cell smFISH revealed high levels of cytokine mRNA expression, indicating a diverse transcription response among T cells.
  • Nuclear accumulation of cytokine-specific mRNAs was observed during activation, while cytoplasmic mRNAs underwent decay through translation-dependent mechanisms.
  • The study indicates that HuR selectively regulates cytokine mRNA, highlighting the complexity of post-transcriptional processes.
  • Using single-cell resolution, this technique offers novel insights into the dynamic regulation of mRNA in human T cells.

Cite This Study

Lattanzio et al. (2025) studied this question.

synapsesocial.com/papers/68ef858cc6a308ba06355519https://doi.org/10.1038/s44318-025-00592-0
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