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November 25, 2025The Journal of ImmunologyOpen Access

Measurement of human immune cells producing the immunosuppressive/cell repair cytokines TGF-b, IL-10 and others achieved using a novel 50-parameter CyTOF intracellular cytokine staining panel 4022

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Authors

LPLaura PolancoLTLauren TraceyMCMichael J. Cohen

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Overview

Novel mass cytometry identifies cytokines in immune cells, revealing targets for drug development and anti-inflammatory therapies.

Key Points

  • This research aims to enhance detection of anti-inflammatory mediators and cell repair processes in human immune responses.
  • Developed a 50-parameter CyTOF panel for immune cell profiling and cytokine detection.
  • Stimulated and stained PBMC to acquire data on the CyTOF XT system.
  • Used PhenoGraph for clustering and opt-SNE for visualizing cellular diversity.
  • Successfully detected IL-5, IL-10, IL-13, and TGF-b-producing immune cells.
  • Identified co-expression patterns with the pro-inflammatory cytokine IFNg.
  • Predicted novel immune regulatory mechanisms may enhance chronic inflammation treatment and vaccine responses.

Cite This Study

Polanco et al. (2025) studied this question.

synapsesocial.com/papers/692502a487af00ed34ac195chttps://doi.org/10.1093/jimmun/vkaf283.1757
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