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December 8, 2025BloodOpen Access

A defined culture model for ex vivo expansion of primary AML cells preserves leukemic stem cells and clonal architecture for functional and therapeutic studies

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Authors

UGUpendar R. GollaKMKentaro MinagawaHZHong Zheng

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Overview

Observed distinct expansion of leukemia stem cells in different cytokine conditions, suggesting improved therapeutic modeling for acute myeloid leukemia.

Key Points

  • Leukemia stem cells preserved in serum-free culture, enabling successful transplantation in mice.
  • CD11b increased significantly across culture conditions, especially in the presence of specific cytokines.
  • Transplantation success varied, with up to 89% engraftment in optimally expanded acute myeloid leukemia cells.
  • Venetoclax testing revealed its ability to induce apoptosis, indicating potential in treating acute myeloid leukemia.

Cite This Study

Golla et al. (2025) studied this question.

synapsesocial.com/papers/69362f574fa91c937236d9fehttps://doi.org/10.1182/blood-2025-3488
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