September 10, 2025BIO-PROTOCOLOpen Access

Assessing Human Treg Suppression at Single-Cell Resolution Using Mass Cytometry

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This protocol quantifies Treg-mediated suppression in autologous PBMCs, indicating high-dimensional phenotyping capabilities.

Treg-mediated suppression is quantified at single-cell resolution, enabling detailed analysis.
The approach uses a 52-marker CyTOF panel to assess phenotypic responses across immune subsets.
Tregs isolated by cell sorting are reintroduced to evaluate suppression in a physiological environment.
This method is compatible with patient samples and can support drug evaluation efforts.

Søndergaard et al. (2025) studied this question.

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