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August 26, 2025MoleculesOpen Access

A Novel Aptamer Selection Strategy for Pseudomonas aeruginosa and Its Application as a Detecting Probe in a Hybrid Lateral Flow Assay

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Authors

TPThu Thao PhamNHNguyễn Thị HuyềnLOLe Hong Oanh

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Overview

Aptamer-based approach shows high specificity for detecting Pseudomonas aeruginosa, indicating potential for rapid diagnostics.

Key Points

  • A hybrid lateral flow immunoassay achieved a visual detection limit of 2.34 × 102 CFU/mL in just 15 minutes.
  • Molecular docking analysis revealed that aptamer T1 exhibited the highest target selectivity for Pseudomonas aeruginosa.
  • The novel whole-cell aptamer selection method enhanced aptamer recovery over traditional microtiter-plate systems.
  • Integrating aptamer T1 into the LFIA replaced conventional detection antibodies, improving the assay's effectiveness.

Cite This Study

Pham et al. (2025) studied this question.

synapsesocial.com/papers/68af7c947567bf4f94ff43a1https://doi.org/10.3390/molecules30173499
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A Novel Aptamer Selection Strategy for <em>Pseudomonas aeruginosa </em>and Its Application as a Detecting Probe in a Hybrid Lateral Flow Assay2025
  2. 2Aptamer Engineering: Strategies for Discovering Functional Nucleic Acids for Next‐Generation Diagnostics and Biosensing2025
  3. 3Enhanced sensitivity detection via orientational labelling of SPA-functionalized magnetic nanoparticles in lateral-flow immunoassays2025
  4. 4Research Progress on Detection Technologies for Pseudomonas aeruginosa2025
  5. 5Simultaneous Detection of Protein and RNA Using Proximity Ligation of Aptamers and Quantitative Polymerase Chain Reaction2025