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August 10, 2025

Simultaneous Detection of Protein and RNA Using Proximity Ligation of Aptamers and Quantitative Polymerase Chain Reaction

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Authors

EZEmil ZaripovCGChu‐Shu GuKHKevin S. Holmes

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Overview

Novel diagnostic platform detects SARS-CoV-2 RNA and N protein simultaneously, highlighting enhanced sensitivity and adaptability for multiplexed diagnostics.

Key Points

  • Simultaneous detection of SARS-CoV-2 RNA and nucleocapsid protein enhances diagnostic capabilities for viral identification.
  • Utilizing a dual-analyte assay, the method combines proximity ligation of aptamers and RT-qPCR for accuracy.
  • Approach involves high-affinity aptamers and Cy5-labeled TaqMan probes, ensuring effective target detection during analysis.
  • This scalable technique may improve rapid diagnostics in clinical settings, particularly for COVID-19 testing.

Cite This Study

Zaripov et al. (2025) studied this question.

synapsesocial.com/papers/68af2974cf1dd9ea359e2ef1https://doi.org/10.26434/chemrxiv-2025-1d2c4-v2
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