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July 26, 2026BiomedicinesOpen Access

Combined lysis and sonication boosts measurable cTnT levels ~34% while slashing processing time.

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Population

Plasma samples from patients with non-ST-segment elevation myocardial infarction, unstable angina, pulmonary…

Comparison

Combined lysis- and sonication-based protocol vs Native, unprocessed samples and a conventional…

Design

Other

Key result

A combined lysis and sonication protocol significantly increased measurable cTnT levels by ~10% to ~34% compared to unprocessed samples, reducing processing time from ~2.5 hours to ~10 minutes.

Authors

YDY. DingDBDominika Bernáth-NagyCHChiara Heß

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Overview

Enables detection of previously missed EV-bound cTnT; extends hs-cTnT assay utility for myocardial injury assessment.

Key Points

  • This research aims to develop a quick and efficient method to detect extracellular vesicle-bound cardiac troponin T using lysis and sonication.
  • Plasma samples were treated with a lysis buffer and sonicated for analysis.
  • Comparisons were made between the lysis-sonication method and a conventional EV isolation method.
  • Sample groups included patients with various cardiac conditions and healthy controls.
  • Post-processing, cTnT levels increased significantly, with a median rise of ~10% to ~34% across cohorts.
  • In NSTEMI, ~15% of plasma cTnT was found to be EV-bound, remaining stable over 72 hours.
  • Processing time for cTnT measurement was reduced from ~2.5 hours to ~10 minutes.

Study Design

Type

Observational

Structured PICO

P
Population
Plasma samples from patients with various cardiovascular conditions and healthy controls used to evaluate a new cTnT quantification method.
E
Exposure
Combined lysis- and sonication-based protocol
C
Comparator
Native, unprocessed samples and a conventional EV isolation method
O
Outcome
Extracellular vesicle-bound cardiac troponin T (cTnT) levels and processing timesurrogate

A novel lysis- and sonication-based protocol allows for rapid quantification of EV-bound cTnT, reducing processing time from 2.5 hours to 10 minutes.

Cite This Study

Ding et al. (2026) conducted an observational in Cardiovascular diseases and healthy controls. Combined lysis- and sonication-based protocol vs. Native, unprocessed samples was evaluated on cTnT levels. A combined lysis and sonication protocol significantly increased measurable cTnT levels by ~10% to ~34% compared to unprocessed samples, reducing processing time from ~2.5 hours to ~10 minutes.

synapsesocial.com/papers/6a65a6b5d3aea3239cd77d65https://doi.org/10.3390/biomedicines14081653
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The actively secreted plasma extracellular vesicle troponin (ASPECT) study: circulating troponin in extracellular vesicles across cardiovascular disease cohorts2026
  2. 2Design and Analytical Evaluation of an Immunoassay for Long Forms of Cardiac Troponin T2025
  3. 3Kinetics of High-Sensitive Cardiac Troponin I in Patients with ST-Segment Elevation Myocardial Infarction and Non-ST Segment Elevation Myocardial Infarction2025
  4. 4Aptamer-Based Gold Nanoparticle Lateral Flow Assay for Rapid Detection of Cardiac Troponin I2025
  5. 5Long forms of cardiac troponin T for myocardial infarction diagnosis: the SuperTROPO study2025 · 1 citations