Synapse
⌘+K
Synapse
PulseExploreJournal ClubResearchersJournals
Instagram
HomeJournal ClubExplore
December 8, 2025Blood

Single-cell genotyping and methylome sequencing (scGEM-seq) resolves cell-states and clonal hierarchies in leukemia

View Full Paper
Ask AI
Bookmark
Share

Authors

KKKyung Lock Kim

Discussion

Loading...

Member takes

Overview

scGEM-seq demonstrates efficient characterization of genetic variants and methylation in leukemia, pointing to clonal heterogeneity in cancer cell lines.

Key Points

  • Malignant cells are accurately classified by scGEM-seq, which resolves leukemia evolution using genetic variants.
  • Study reveals issues with low doublet rates, enhancing genotyping for cancer genes with a mean of around 13% using hybrid capture.
  • Analysis combines single-cell methods including hybrid capture and amplicon sequencing to assess myeloid neoplasms with varied patient blast counts.
  • The flexible cost-effective platform may enable better understanding of clonal heterogeneity and minimal residual disease.

Cite This Study

Kyung Lock Kim (2025) studied this question.

synapsesocial.com/papers/69362f604fa91c937236dcafhttps://doi.org/10.1182/blood-2025-942
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Bioinformatic methods for GRC scRNAseq poster v12025
  2. 2Replacing FISH with a comprehensive integrated approach for tumor genotyping and immune monitoring in multiple myeloma: The flagship study2025 · 1 citations
  3. 3High-throughput single-cell methylation resolves epigenetic states in hematopoietic differentiation and age-related clonal expansions2025
  4. 4Abstract P17: FocuSCOPE: A Multi-omics Solution to Simultaneously Analyse Genetic Variants and Transcriptome in Single Cells in Leukemia2025
  5. 5Detecting Somatic Mutations in Rare Clones using Single Cell Multi-Omics2025