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December 8, 2025Blood

Pre-treatment immune profiling by flow cytometry and single-cell transcriptomics identifies distinct features in B and T cells in rituximab responders and non-responders

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Authors

AAAlicia K. AuAKAkhil KheraAMAmna Malik

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Overview

Flow cytometry shows distinct immune profiles for rituximab responders and non-responders, indicating biomarkers for treatment response.

Key Points

  • To identify pre-treatment immune signatures associated with rituximab response in patients with immune thrombocytopenia.
  • Conducted multi-parameter flow cytometry and single-cell RNA and T cell receptor sequencing on patients before treatment.
  • Isolated peripheral blood mononuclear cells from blood samples.
  • Performed deep immunophenotyping of immune subsets including T, B, and natural killer cells.
  • Identified distinct immune signatures between complete responders and non-responders to rituximab.
  • Non-responders exhibited heightened immune activation and increased HLA-DR expression on T cells.
  • Complete responders showed enhanced B cell receptor signaling and functional activation.

Cite This Study

Au et al. (2025) studied this question.

synapsesocial.com/papers/69362f364fa91c937236d307https://doi.org/10.1182/blood-2025-734
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Also Consider

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  1. 1Rituximab beats the odds in ITP? a 5-year national showdown against TPO-ras2025
  2. 2Single-cell dissection of pathogenic T-cell response to tacrolimus in immune thrombocytopenia2025
  3. 3Immune landscape characterization of relapsed/refractory B-cell lymphoma patients treated with CD19 CAR-T cell therapy2025
  4. 4Urine proteomics uncovers biomarker candidates for early identifying rituximab beneficiaries in paediatric steroid‐resistant immune thrombocytopenia2025 · 1 citations
  5. 5Pre-existing cytotoxic capacity of CD8+ and CD4+ T cells is a hallmark of richter transformation and favors response to anti-PD-1 therapy2025