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December 8, 2025NutrientsOpen Access

A Low-Cost, High-Fat Diet Effectively Induces Obesity and Metabolic Alterations and Diet Normalization Modulates Microbiota in C57BL/6 Mice

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Authors

MPMaria do Carmo Gouveia Pelúzio

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Overview

Diet normalization reduces adiposity in C57BL/6 mice, suggesting metabolic and microbial changes may persist after HFD withdrawal.

Key Points

  • This research aims to evaluate a cost-effective high-fat diet model in mice and explore changes in metabolism and gut microbiota during dietary normalization.
  • C57BL/6 mice were fed a low-cost high-fat diet (HFD) for 12 weeks (Phase 1) and then switched to a normal diet for another 12 weeks (Phase 2).
  • Body weight and adiposity were measured, alongside blood glucose and biochemical parameters.
  • Fecal samples were analyzed for short-chain fatty acids and microbiota composition using 16S rRNA sequencing.
  • The HFD significantly increased body weight and adiposity compared to the standard diet.
  • After dietary normalization, previously obese mice showed a higher Lee index and unique metabolic profiles.
  • Fecal analyses indicated changes in short-chain fatty acid levels, with an increase in butyrate in HFD mice.

Cite This Study

Maria do Carmo Gouveia Pelúzio (2025) studied this question.

synapsesocial.com/papers/693624c34fa91c937236cb2ehttps://doi.org/10.3390/nu17233806
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