September 10, 2025FoodsOpen Access

A Simple, Rapid, and Contamination-Free Ultra-Sensitive Cronobacter sakazakii Visual Diagnostic Platform Based on RPA Combined with CRISPR/Cas12a

View Full Paper

Ask AI

Bookmark

Authors

Discussion

This novel one-pot method detects Cronobacter sakazakii using CRISPR/Cas12a and RPA, avoiding aerosol contamination.

The developed PTIT method achieves detection limits of 4.65 CFU/mL in milk powder samples, ensuring high sensitivity.
Sensitivity was confirmed for genomic DNA at 10−4 ng/μL and target DNA at 1.43 copies/μL, highlighting its precision.
This one-pot nucleic acid detection strategy simplifies the process without specialized devices, overcoming contamination issues.
The method demonstrates potential for rapid point-of-care testing, offering a significant advancement in food safety diagnostics.

Liu et al. (2025) studied this question.

View Full Paper

Ask AI

Bookmark