This analysis demonstrates Cascade-CRISPR improved detection sensitivity for Toxoplasma gondii and Listeria monocytogenes, indicating its potential in rapid diagnostics.
Key Points
Cascade-CRISPR achieves detection sensitivity of 10 fM for foodborne pathogens, minimizing false positives.
Results show strong performance in real food samples, demonstrating its viability for on-site testing.
The method utilizes a hairpin DNA amplifier, significantly enhancing detection without pre-amplification.
High sensitivity and rapid results underscore potential for safer food screening practices.],