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September 5, 2025Scientific JournalOpen Access

Evaluation of Antioxidant Activity of Methanol Extract of Neem Leaves (Azadirachta indica) Using DPPH, ABTS, and FRAP Methods

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Authors

NANoer AiniATAndri Tilaqza

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Overview

Study assesses antioxidant activity of neem leaf extract via DPPH, ABTS, and FRAP methods, indicating its potential health benefits.

Key Points

  • The methanolic extract of neem leaves showed strong antioxidant activity in the DPPH assay.
  • DPPH assay resulted in an IC₅₀ value of 54.91 ppm, indicating effective free radical scavenging.
  • Extraction used Ultrasound-Assisted Extraction, assessing multiple methods to evaluate antioxidant capacity.
  • Findings suggest the primary antioxidant mechanism of neem extract relies on hydrogen atom transfer rather than electron transfer.

Cite This Study

Aini et al. (2025) studied this question.

synapsesocial.com/papers/68c239a3b210217d6477cadehttps://doi.org/10.56260/sciena.v4i5.267
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  1. 1Evaluation of Antioxidant Activity of Methanol Extract of Neem Leaves (Azadirachta indica) Using DPPH, ABTS, and FRAP Methods2025
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  3. 3Phytochemical Profile and Antioxidant Activity of Ethanolic Extracts from Selected Wild Medicinal Plants: Azadirachta indica, Acalypha indica, and Muntingia malabura2025
  4. 4Inhibitory activity of an ethanolic extract from Azadirachta indica (Neem) on Fusarium sp.2025
  5. 5Phytochemical screening of bioactive compounds and antioxidant activity of methanol extract from Melia Azedarach L. fruit: Exploring for antidiabetic potential2025