August 16, 2025

Analysis of discordant results between multiplex fluorescence PCR-capillary electrophoresis for microsatellite instability (MSI) detection and immunohistochemistry (IHC) for mismatch repair (MMR) protein expression in gastrointestinal adenocarcinoma.

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Discussion

Retrospective analysis showed discordant results in gastrointestinal adenocarcinoma, indicating potential sampling issues and method limitations.

Discordant results occurred in 15 out of 511 gastrointestinal adenocarcinomas, highlighting technical challenges.
In gastric cancers, the discordance rate was 7.5%, significantly higher than the 0.9% observed in colorectal cancers (P＜0.001).
A retrospective analysis combined multiplex fluorescence PCR-capillary electrophoresis and immunohistochemistry for accuracy.
Validation with next-generation sequencing resolved some discrepancies, emphasizing the need for a systematic evaluation approach.

Gu et al. (2025) studied this question.

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Retrospective analysis showed discordant results in gastrointestinal adenocarcinoma, indicating potential sampling issues and method limitations.