What is the clinical evidence from this study?

Population: Angiotensin II-induced hypertensive injury and cardiomyocyte hypertrophy. Intervention: Cardojith (CJ) vs. Angiotensin II without Cardojith treatment. Primary outcome: Cell viability, oxidative stress parameters, hypertrophic/inflammatory mediators, gene expression, and apoptosis.

What does this research mean for the field?

The polyherbal formulation Cardojith protects against Angiotensin II-induced cardiomyocyte hypertrophy and injury by attenuating oxidative stress, inflammation, apoptosis, and p38 MAPK signaling. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

September 5, 2026Applied Biochemistry and Biotechnology

Protective Effect of a Polyherbal Formulation (Cardojith) Against Angiotensin II-induced Hypertension and Cardiomyocyte Hypertrophy Via Modulation of Oxidative Stress and MAPK Signalling

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Key result

Cardojith attenuates Ang II-induced cardiomyocyte pathology by reducing oxidative stress, inflammation, and apoptosis.

Why the study?

Does Cardojith protect against Angiotensin II-induced hypertensive injury and cardiomyocyte hypertrophy in H9c2 cells?

Key Points

To evaluate the protective efficacy and molecular mechanisms of the polyherbal formulation Cardojith against Angiotensin II-induced oxidative stress, inflammation, and hypertrophy in H9c2 cardiomyocytes.
Exposed H9c2 cardiomyocytes to Angiotensin II (Ang II) with or without Cardojith (CJ) treatment.
Quantified cell viability by MTT assay, apoptosis by AO/EtBr staining, oxidative stress parameters (ROS, TBARS, SOD, CAT), and inflammatory/hypertrophic mediators (ANP, BNP, IL-6, TNF-α).
Measured AT1R, NF-κB, and COX-2 gene expression via RT-PCR and p38 MAPK phosphorylation through Western blotting.
Ang II exposure significantly decreased cell viability, elevated ROS, TBARS, apoptosis, and hypertrophic markers (ANP, BNP, IL-6, TNF-α), while upregulating AT1R, NF-κB, COX-2, and p38 MAPK phosphorylation.
Cardojith treatment restored SOD and CAT antioxidant enzyme activities, reduced ROS and lipid peroxidation, decreased apoptotic cell death, and normalized ANP and BNP levels.
Cardojith downregulated the expression of AT1R, NF-κB, and COX-2 and suppressed p38 MAPK activation, mitigating pro-hypertrophic and inflammatory signaling.

Structured PICO

Does Cardojith protect against Angiotensin II-induced hypertensive injury and cardiomyocyte hypertrophy in H9c2 cells?

Population

In vitro study of H9c2 cardiomyocytes exposed to Angiotensin II to evaluate the protective effects of the polyherbal formulation Cardojith.

Intervention

Polyherbal formulation Cardojith (CJ)

Comparator

Angiotensin II exposure without Cardojith treatment

Outcome

Cell viability, oxidative stress parameters (ROS, TBARS, SOD, CAT), hypertrophic/inflammatory mediators (ANP, BNP, IL-6, TNF-α), gene expression (AT1R, NF-κB, COX-2), p38 MAPK activation, and apoptosissurrogate

Cardojith demonstrates cardioprotective effects in vitro by attenuating Angiotensin II-induced oxidative stress, inflammation, apoptosis, and hypertrophic signaling in H9c2 cardiomyocytes.

Discussion

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Implication

May protect H9c2 cells from Ang II injury in vitro; leaves open clinical translation in hypertension.

Cite This Study

M et al. (2026) studied Angiotensin II-induced hypertensive injury and cardiomyocyte hypertrophy. Cardojith (CJ) vs. Angiotensin II without Cardojith treatment was evaluated on Cell viability, oxidative stress parameters, hypertrophic/inflammatory mediators, gene expression, and apoptosis. Cardojith attenuated Ang II-induced pathological changes in H9c2 cardiomyocytes by reducing oxidative stress, inflammation, and apoptosis, and downregulating key signaling pathways.

synapsesocial.com/papers/6a9c00e86b95aff0620ec807 https://doi.org/10.1007/s12010-026-05905-5

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Protective Effect of a Polyherbal Formulation (Cardojith) Against Angiotensin II-induced Hypertension and Cardiomyocyte Hypertrophy Via Modulation of Oxidative Stress and MAPK Signalling

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Protective Effect of a Polyherbal Formulation (Cardojith) Against Angiotensin II-induced Hypertension and Cardiomyocyte Hypertrophy Via Modulation of Oxidative Stress and MAPK Signalling

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider