PulseExploreJournal ClubResearchersJournals
Instagram
HomeJournal ClubExplore
Synapse
⌘+K
Synapse
September 5, 2026International Journal of Molecular SciencesOpen Access

Elevated Prorenin Induces Podocyte Injury and Glomerular Fibrosis in cyp1a1-Prorenin Transgenic Rats

View Full Paper
Ask AI
Bookmark
Share

Why the study?

Does elevated prorenin induce podocyte injury and glomerular fibrosis in a transgenic rat model?

Population

cyp1a1-prorenin transgenic rats and wild-type rats

Comparison

Diets containing indole-3-carbinol for 4 weeks… vs Wild-type rats maintained on a normal diet

Design

Preclinical, randomized to receive diets

Follow-up

4 to 6 weeks

Key result

Inducing elevated prorenin with 0.05%, 0.15%, or 0.3% indole-3-carbinol in transgenic rats dose-dependently increased mean arterial pressure, podocyte injury, and glomerular fibrosis.

Authors

CGChunyan GuXLXia LiuJWJie Wu

Discussion

Loading...

Member takes

Overview

Should not change clinical practice; extends experimental evidence for prorenin in glomerular injury but requires human validation.

Key Points

  • To determine whether elevated plasma prorenin directly promotes podocyte dysfunction, albuminuria, and glomerular fibrosis using an inducible transgenic rat model.
  • Randomized cyp1a1-prorenin transgenic rats to receive diets containing 0.05%, 0.15%, or 0.3% of the gene activator indole-3-carbinol (I3C) for 4 weeks alongside wild-type controls.
  • Administered 6-week treatment regimens of either amlodipine or enalapril to evaluate blood pressure control and suppression of inflammatory and oxidative pathways.
  • Induction of prorenin caused dose-dependent increases in mean arterial pressure and urinary albumin excretion while reducing podocyte counts and slit diaphragm protein expression.
  • Prorenin elevation stimulated profibrotic factors and glomerular fibrosis, which were only partially attenuated by enalapril or amlodipine through NF-κB and Nox2 pathway suppression.

Structured PICO

Does elevated prorenin induce podocyte injury and glomerular fibrosis in a transgenic rat model?

P
Population
cyp1a1-prorenin transgenic rats randomized to receive diets containing increasing concentrations of indole-3-carbinol for 4 weeks.
I
Intervention
Diets containing indole-3-carbinol (I3C; 0.05%, 0.15%, or 0.3%) for 4 weeks to induce prorenin expression, followed by treatment with amlodipine or enalapril for 6 weeks
C
Comparator
Wild-type rats maintained on a normal diet
O
Outcome
Podocyte injury (podocyte number, slit diaphragm protein expression, foot process effacement, podocyte hypertrophy) and glomerular fibrosissurrogate

Elevated prorenin promotes podocyte injury and glomerular fibrosis through both angiotensin II-dependent and independent pathways, highlighting its pathogenic role in nephropathy.

Cite This Study

Gu et al. (2026) studied Podocyte injury and glomerular fibrosis. Indole-3-carbinol (I3C) diet vs. Wild-type rats on a normal diet was evaluated on Podocyte injury and glomerular fibrosis. Inducing elevated prorenin with 0.05%, 0.15%, or 0.3% indole-3-carbinol in transgenic rats dose-dependently increased mean arterial pressure, podocyte injury, and glomerular fibrosis.

synapsesocial.com/papers/6a9bd4346b95aff0620eba85https://doi.org/10.3390/ijms27177888
View Full Paper
Ask AI
Bookmark
Share