Randomized trial demonstrates bile acid metabolism's prognostic significance in colorectal cancer, suggesting new therapeutic avenues.
Key Points
This research aims to understand the prognostic role of bile acid metabolism in colorectal cancer (CRC) and its relationship with host genetics and gut microbiota.
Combined bulk transcriptomics, single-cell, and spatial transcriptomics along with microbiome analysis.
Validated findings using qRT-PCR on paired tumor and normal tissue samples from patients.
Developed a 15-gene prognostic signature based on transcriptomic analysis.
The bile acid metabolism pathway showed significant transcriptional suppression in CRC tumors.
A 15-gene prognostic signature correlated with overall survival across five independent cohorts (exact figures not specified).
Spatial transcriptomics revealed enrichment of the pathway's activity in metastatic stromal niches and specific tumor microenvironments.