What question did this study set out to answer?

This research investigates how glucose metabolism affects glycoprotein maturation and monocyte adhesion in human aortic valve cells.

June 14, 2026AJP Heart and Circulatory Physiology

Glycolysis and hexosamine biosynthesis regulate inflammatory protein expression and maturation and key steps of monocyte recruitment by human aortic valve cells

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Why the study?

Does inhibiting glycolysis and hexosamine biosynthesis reduce inflammatory protein expression and monocyte recruitment in human aortic valve cells?

Key Points

This research investigates how glucose metabolism affects glycoprotein maturation and monocyte adhesion in human aortic valve cells.
Conducted real-time metabolic analysis of valve endothelial cells (VECs) and valve interstitial cells (VICs).
Examined the effects of inhibiting glucose metabolism on inflammatory protein expression and monocyte adhesion.
Evaluated the role of hexosamine biosynthesis in adhesion molecule maturation under inflammatory conditions.
VECs exhibited higher glycolytic activity compared to VICs; glucose inhibition diminished inflammatory protein expression (interleukin-6 and cyclooxygenase-2).
Reduced adhesion molecule maturation was observed when hexosamine biosynthesis was inhibited, correlating with impaired monocyte recruitment.
Under shear stress, inhibition of glycolysis with 2-DG and DON affected monocyte rolling and adhesion to VECs.

Structured PICO

Does inhibiting glycolysis and hexosamine biosynthesis reduce inflammatory protein expression and monocyte recruitment in human aortic valve cells?

Population

Human aortic valve cells (valve interstitial cells [VICs] and valve endothelial cells [VECs])

Intervention

Inhibition of glucose metabolism (2-DG), hexosamine biosynthesis (DON), and N-glycosylation (tunicamycin)

Comparator

Uninhibited cells under inflammatory stimuli

Outcome

Inflammatory protein expression (IL-6, COX-2), adhesion molecule maturation (ICAM-1, VCAM-1), and monocyte adhesion/rollingsurrogate

Glycolysis and hexosamine biosynthesis are crucial for inflammatory protein maturation and monocyte recruitment in human aortic valve cells, highlighting potential metabolic targets for early-stage calcific aortic valve disease.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Should not change valve disease care; leaves open whether glycolytic inhibition will prove therapeutic in patients.

Cite This Study

Sánchez‐Bayuela et al. (2026) studied this question.

synapsesocial.com/papers/6a2e4632b1cc60ccdea8af6e https://doi.org/10.1152/ajpheart.00974.2025

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Glycolysis and hexosamine biosynthesis regulate inflammatory protein expression and maturation and key steps of monocyte recruitment by human aortic valve cells

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Glycolysis and hexosamine biosynthesis regulate inflammatory protein expression and maturation and key steps of monocyte recruitment by human aortic valve cells

Key Points

Structured PICO

Discussion

Implication

Cite This Study

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