Observational analysis reveals immune cells and resistance mechanisms in bone marrow microenvironment, indicating new biomarkers for treatment response.
Key Points
Leukemia-specific cellular neighborhoods dramatically altered the bone marrow microenvironment, indicating potential treatment resistance.
Utilizing CODEX and MERFISH technologies, a total of over 1 million high-quality cells were imaged and analyzed.
Machine-learning classification distinguished between myeloid blast differentiation states and regulatory immune cells within the BMME.
Findings suggest that specific immune and stromal components in the bone marrow predict clinical outcomes in NPM1-mutant AML.