Analysis reveals unique immune activation profiles in ex vivo NK cells with different cytokine treatments, suggesting optimized methodologies for cancer immunotherapy.
Key Points
Expanded NK cells exhibit distinct immunophenotypes characterized by elevated activation markers and variable CD16 expression.
Significant differences in gene expression and global DNA methylation patterns were noted across NK cell expansion methods.
Single-cell sequencing identified unique clusters of activated NK cells with enrichment in immune activation pathways.
This work highlights the diverse signaling pathways pertinent for optimizing ex vivo NK cell expansion for therapeutic applications.