December 8, 2025BloodOpen Access

Lipid nanoparticle-mediated epitope editing in human HSPCs enables resistance to CD45-directed CAR-T cell therapy

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Authors

EGElahe Kamali GhaheParker Institute for Cancer Immunotherapy DTDavin TafuriParker Institute for Cancer Immunotherapy RDRujul Jagdish DeolikarParker Institute for Cancer Immunotherapy

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Implication

Observational analysis improved gene editing efficiency and cell viability in human HSPCs, highlighting LNP as a promising alternative to electroporation.

Key Points

Editing via lipid nanoparticles achieved comparable efficiency to electroporation, improving cell viability significantly.
Colony-forming units showed no difference between lipid nanoparticle and electroporation delivery methods, ensuring functionality of edited cells.
Transplantation of edited HSPCs demonstrated successful long-term engraftment and multi-lineage differentiation in immunodeficient mice.
Both delivery methods effectively protected HSPCs from CD45-directed CAR-T cell-mediated cytotoxicity, supporting clinical applicability.

Cite This Study

Ghahe et al. (2025) studied this question.

synapsesocial.com/papers/69362f3a4fa91c937236d43a https://doi.org/10.1182/blood-2025-1048

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Implication

Observational analysis improved gene editing efficiency and cell viability in human HSPCs, highlighting LNP as a promising alternative to electroporation.

Lipid nanoparticle-mediated epitope editing in human HSPCs enables resistance to CD45-directed CAR-T cell therapy

Discussion

Member takes

Implication

Key Points

Cite This Study

Lipid nanoparticle-mediated epitope editing in human HSPCs enables resistance to CD45-directed CAR-T cell therapy

Discussion

Member takes

Implication

Key Points

Cite This Study

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