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December 8, 2025BloodOpen Access

Soluble factors in the AML secretome drive macrophage polarization toward an M2-like state promoting an immunosuppressive microenvironment

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Authors

MBMaddalena BenettonMSMonica Sandri

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Overview

Proteomic analysis reveals secretome of AML-MSCs promotes macrophage polarization and immunosuppression in acute myeloid leukemia.

Key Points

  • Investigate how soluble factors in the AML secretome influence macrophage polarization.
  • Co-cultured AML-MSCs with primary AML blasts for 7, 14, and 21 days.
  • Performed proteomic analysis by mass spectrometry to identify differentially secreted proteins.
  • Measured PCP levels using ELISA to investigate immunosuppressive properties of AML-Ms.
  • 9 proteins specifically upregulated in AML niches are associated with cancer progression.
  • PCP increases macrophage polarization towards an M2-like phenotype, enhancing immunosuppression.
  • PCP promoted reduced T-cell activation and proliferation in co-culture experiments.

Cite This Study

Benetton et al. (2025) studied this question.

synapsesocial.com/papers/693624dd4fa91c937236d228https://doi.org/10.1182/blood-2025-1407
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