Single-cell analysis uncovers distinct differentiation trajectories in leukemic stem cells across AML epigenetic subgroups, indicating potential for targeted therapies.
Key Points
The study aims to explore the differentiation trajectories and transcriptional programs in epigenetic subgroups of acute myeloid leukemia (AML).
Performed single-cell RNA and ATAC-seq on 36 AML samples across 16 epigenomic subgroups
Conducted computational analyses including cell clustering, pseudotime inference, and TF activity analysis
Refined differentiation states and evaluated leukemic stem cell signatures through multi-platform profiling.
Identified distinct leukemic cell clusters that co-cluster by epigenetic subgroup
Revealed divergent differentiation arrest patterns among subgroups, even in genetically similar AML cases
Demonstrated that TFs exhibited subgroup-specific activation dynamics during differentiation trajectories.
Cite This Study
Liew-Littorin et al. (2025) studied this question.