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This research aims to elucidate the cellular composition and signaling mechanisms within the hematopoietic niche.

December 8, 2025BloodOpen Access

Spatial transcriptomics identified dynamic and spatial-resolved niche cell and signal architecture for hematopoietic stem cell specification

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This research aims to elucidate the cellular composition and signaling mechanisms within the hematopoietic niche.
Utilized spatial transcriptomics and single-cell RNA sequencing to analyze AGM regions at specific embryonic days.
Examined interactions between niche cells and pre-HSCs via ligand-receptor pairing using CellChat.
Validated findings with MERFISH to visualize spatial distributions and cell identities.
Identified N-cad+ mesenchymal stromal cells as key niche components influencing HSC specification.
Demonstrated dynamic signaling interactions including Dlk1-Notch1 and Tgfβ1-Tgfβr1 across developmental stages.
Confirmed a unique layer of mesenchymal cells adjacent to pre-HSCs, critical for their development.

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Spatial transcriptomics identifies niche signaling and mesenchymal stromal cell roles in hematopoietic stem cell specification.

Mao et al. (2025) studied this question.

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