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December 8, 2025Biochemistry (Moscow)Open Access

Reporter System for Detection of G-Quadruplexes in the Human Telomerase Reverse Transcriptase Gene Promoter Region

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Authors

ЕКЕ. А. Кубарева

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Overview

This research demonstrates G-quadruplex formation in the hTERT promoter, suggesting implications for anti-tumor therapy.

Key Points

  • The study aims to explore the formation and stability of G-quadruplexes in the hTERT gene promoter region.
  • Utilized plasmid constructs with fluorescent protein genes to study G4 formation in the hTERT promoter region.
  • Employed DNA polymerase stop assay to demonstrate G4 formation.
  • Investigated the effects of G228A and G250A substitutions on G4 stability under physiological conditions.
  • Confirmed G-quadruplex formation in the central hTERT promoter region of plasmid constructs.
  • Identified that ligands BRACO19 and TMPyP4 stabilize the G4 structure at concentrations of 5-25 μM.

Cite This Study

Е. А. Кубарева (2025) studied this question.

synapsesocial.com/papers/693624ce4fa91c937236cd69https://doi.org/10.1134/s0006297925601753
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