Detection of ARGs from Gram-Negative Bacteria in Positive Blood Cultures Using a Microarray-Based System: Towards a Molecular Antibiotic Susceptibility Assay
This research shows effective detection of ARGs in blood culture from gram-negative bacteria, suggesting rapid diagnostics may enhance antimicrobial stewardship.
Key Points
The study aims to evaluate a microarray-based system for detecting antimicrobial resistance genes in positive blood cultures.
Evaluated HybriSpot12 PCR AUTO (HS12a) and MDR Direct Flow Chip (MDR-FC) Kit for ARG detection.
Analysed 111 Gram-negative bacterial isolates from positive blood cultures.
Compared diagnostic performance against whole-genome sequencing results.
Demonstrated perfect agreement for detecting macrolide resistance across bacterial species.
High agreement found for resistance to sulfonamides and β-lactams.
Moderate agreement for aminoglycoside resistance genes, with discrepancies in specific isolates.