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December 5, 2025CancersOpen Access

CRISPR-Cas9 Genome and Double-Knockout Screening to Identify Novel Therapeutic Targets for Chemoresistance in Triple-Negative Breast Cancer

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Authors

SSShuai ShaoSLShangjia LiYHYang Huo

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Overview

Genome-wide screening identified 96 and 93 genes for cisplatin and doxorubicin in triple-negative breast cancer, suggesting potential therapeutic targets to combat chemoresistance.

Key Points

  • Genome-wide screening revealed 96 and 93 genes that could re-sensitize TNBC to cisplatin and doxorubicin, respectively.
  • The analysis focused on the MDA-MB-231 cell line, showing its significant role in studying chemoresistance.
  • CRISPR-Cas9 genome screening and double-knockout techniques were employed to explore cancer pathways in TNBC.
  • Findings suggest that targeting synthetic lethal gene pairs may provide a new strategy to overcome chemoresistance.

Cite This Study

Shao et al. (2025) studied this question.

synapsesocial.com/papers/6932313d8e51979591dcefc2https://doi.org/10.3390/cancers17233876
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