A novel reversed phase high performance liquid chromatography approach for stability-indicating simultaneous analysis of brimonidine tartrate and timolol maleate
Novel method validation demonstrates stability for brimonidine tartrate and timolol maleate in ophthalmic quality control, highlighting improved accuracy and robustness.
Key Points
The method provides a simultaneous analysis of brimonidine tartrate and timolol maleate in ophthalmic formulations, ensuring quality control.
Achieved excellent accuracy between 98.71% and 101.10% for both active ingredients, with a robustness yielding RSDs below 2%.
Analytical separation was conducted via chromatographic methods under various forced degradation conditions to test stability fully.
The findings indicate a balance between rigorous analysis and green analytical chemistry attributes that promotes sustainable practices.