December 4, 2025AntibioticsOpen Access

Evaluation of the Quantitative and Structural Antimicrobial Activity of Thymol, Terpinen-4-ol, Citral, and E-2-Dodecenal, Antibiotic Molecules Derived from Essential Oils

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The study demonstrates significant antimicrobial activity at low concentrations for thymol and citral, effective against various microorganisms.
Quantitative measures reveal that the minimum inhibitory concentrations for the tested essential oils are below 500 µg/mL or 0.05% w/w.
Methods included micro dilution assays to establish activity levels, alongside Scanning Electron Microscopy to visualize cell damage.
Results indicate that essential oils could serve as natural markers for antimicrobial efficacy, with potential applications in antibiotic development.

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Evaluation shows strong antimicrobial activity of thymol and citral against microorganisms, suggesting potential as natural antibiotics.

Noriega et al. (2025) studied this question.

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