Analysis reveals that microglial cells show altered density and morphology in the pineal gland of male rats, indicating adaptations over light:dark cycles.
Key Points
Microglia exhibited altered morphology at night, showing enhanced phagocytic capacity and smaller size, suggesting adaptive responses.
Density and spatial distribution of microglial cells did not vary with time of day in younger rats, according to ZT6 and ZT18 measurements.
Immunofluorescence staining validated the presence of markers like CD68 and PAX6, highlighting specific cellular interactions.
Findings suggest that microglia in the pineal gland adapt with age, affecting their function in nocturnal melatonin production.