Investigating phylogenetic trees built from paired heavy and light chain sequences, revealing improved accuracy and reproducibility with maximum likelihood methods.
Key Points
To explore strategies for inferring B cell phylogenies from paired heavy and light chain sequences.
Analyzed paired heavy and light chain BCR sequences.
Compared accuracy of phylogenetic trees built using different methods.
Incorporated single-cell sequencing data alongside bulk data.
Incorporating light chains improved tree accuracy and reproducibility.
Maximum likelihood methods were effective in correcting biased branch length estimates.
The benefits of using paired sequences exceeded the variations among tree-building methods.