Experiment reveals FoxP3 loss does not impair suppressive function for hCD8 iTregs in peripheral blood, suggesting resilience under destabilizing conditions.
Key Points
This research aims to determine the necessity of FoxP3 for the generation and function of human CD8 induced regulatory T cells (iTregs).
Used single cell RNA-sequencing to analyze hCD8 iTregs from peripheral blood
Evaluated CD4 Treg marker expression including CTLA-4
Tested FoxP3 gene manipulation in mature PB CD8 T cells
hCD8 iTregs express canonical CD4 Treg markers like CTLA-4
hCD8 iTregs retain suppressive function even with FoxP3 loss
Gene-set enrichment analysis showed significant overlap with CD4 Treg markers