Optimization and Comparative Analysis of Phenol-Chloroform vs Salting-Out DNA Extraction Methods from Human Blood and Saliva Tissues: Assessing Yield, Purity, and Suitability for Downstream Molecular Applications
Laboratory analysis of DNA yield and purity from human blood and saliva shows phenol-chloroform results superior to salting-out, indicating suitability for molecular applications.
Key Points
Phenol-chloroform extraction produced significantly higher DNA concentrations from blood and saliva, with yields of ~308 ng/µL from blood.
Spectrophotometric analysis revealed A260/A280 ratios indicating superior purity for DNA extracted via phenol-chloroform compared to salting-out.
Comparative laboratory analysis involved 60 human samples and assessed both methods for yield, purity, and suitability.
Biosafety concerns with organic solvents support the routine use of salting-out, especially in resource-limited settings.