Biosynthesis of Silver Nanoparticles from Streptomyces rochei and its Antibacterial Efficacy against Bacterial Pathogens

The unfolding of bacterial drug resistance poses a significant threat to public health globally, requiring novel and effective antimicrobial strategies. Knowing the necessity of this situation, the current study explores the biosynthesis of silver nanoparticles as potent antibacterial agents. The biosynthesis was explored by a green approach mediated by the endophytic actinomycete Streptomyces rochei, 16S rRNA sequencing helped identification of the actinomycete and assigned the GenBank accession number ON142045. The synthesized silver nanoparticles (AgNPs) were characterized for their formation by using various techniques, which included FE-SEM, EDS, FT-IR, UV-Vis spectroscopy, and DLS. UV-Vis analysis confirmed nanoparticle formation with an absorption peak at 400 nm. The FT-IR analysis shows existence of multiple functional groups such as O–H, C–N, and C=C, which explains biomolecules in nanoparticle stabilization. The biosynthesized AgNPs exhibited notable antimicrobial and bactericidal activity against both MTCC strains and clinically isolated Gram-negative bacterial pathogens. The above results show that the biosynthesized AgNPs possess significant potential as alternative antibacterial agents, offering a promising approach for combating multidrug-resistant bacterial infections. Utilizing endophytic actinomycetes for nanoparticle synthesis promotes environmentally sustainable methods while offering promising opportunities for biomedical use, particularly in antimicrobial treatments. This study highlights the effectiveness of biosynthesized silver nanoparticles in inhibiting bacterial growth, underscoring their potential role in addressing the ongoing challenge of antibiotic resistance through green nanotechnology-based solutions.