Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
October 9, 2025Circulation Research

Abstract Thu099: Single-Cell RNA-seq Analysis Reveals a Two-Step Mechanism for TBX18-Mediated Reprogramming of Ventricular Myocytes into Pacemaker Cells

View Full Paper
Ask AI
Bookmark
Share

Authors

JFJinqi FanJLJing LengHHHannah Hardeo

Discussion

Loading...

Member takes

Overview

Single-cell rna sequencing reveals two-step process for converting cardiac cells, suggesting new pathways for cardiac therapy.

Key Points

  • TBX18 reprogramming of ventricular myocytes results in 19.4% of cells exhibiting pacemaker characteristics by day 14.
  • Single-cell rna sequencing showed a significant increase in pacemaker-like ion channel gene expression in TBX18-treated cells.
  • Transcriptional analysis revealed Klf4 and Junb are critical for the reprogramming of ventricular myocytes into pacemaker cells.
  • Inhibition of TGF-β signaling and activation of Wnt signaling were found to impair iPM induction, highlighting potential therapeutic targets.

Cite This Study

Fan et al. (2025) studied this question.

synapsesocial.com/papers/68e80eb363e2e2f707877dc5https://doi.org/10.1161/res.137.suppl_1.thu099
View Full Paper
Ask AI
Bookmark
Share