Study isolates fungi causing otomycosis and demonstrates the antifungal effects of phenolic extract, highlighting its potential treatment applications.
Background: Otomycosis is a superficial fungal infection that often involves the pinna and auditory canal. It is a pathologic condition, Aspergillus is the most common fungal species. It is common worldwide, characterized by inflammation, pruritis, scaling, and otalgia. Objectives: This study aimed to isolate and identify some fungi cause of otomycosis and treatment by crude phenolic extract of Agaricus bisporus as an antifungal. Materials and Methods: Ear samples were obtained from 110 patients with otomycosis and verified by reverse transcriptase polymerase chain reaction (PCR). High-performance liquid chromatography (HPLC) results showed the presence of six biologically active compounds in A. bisporus . The active substance (phenols) was extracted from the mushroom A. bisporus and the concentrations were prepared (6.25, 12.5, and 25 mg/mL) and then tested against ear fungi Aspergillus niger , Aspergillus flavus , and Penicillium using evaluation of the antifungal activity in the extracts and the fungal growth inhibition was calculated individually. Results: The results showed that the inhibitory activity of the phenolic extract against A. niger , A. flavus , and Penicillium at a concentration of 6.25 mg/mL showed the lowest inhibition activity, as the diameter of inhibition was (1.63 ± 0.18 cm), while the concentration of 25 mg/mL showed the highest inhibition activity, as the diameter of inhibition was (1.17 ± 0.08) compared to the control (8.67 ± 0.33 cm). Conclusion: The crude phenolic extract showed inhibition for fungi that cause otomycosis.
No takes yet. Share an insight, caveat, or question.
Hussein et al. (2025) studied this question.