September 26, 2025Parasites & VectorsOpen Access

18S/28S rDNA metabarcoding identifies Cryptosporidium parvum and Blastocystis ST1 as the predominant intestinal protozoa in hospital patients from Changchun, Northeast China

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Metabarcoding identifies prevalence of Cryptosporidium parvum and Blastocystis ST1 in hospital patients, highlighting food-borne risks.

Cryptosporidium parvum was detected in 7 pools, with a true prevalence of 2.14%, indicating its emergence as a zoonotic threat.
In total, 6.1 million reads were analyzed, with only 1.65% mapping to parasites, underscoring significant primer bias.
The study utilized 360 pooled fecal samples, applying metabarcoding to explore the diversity of intestinal protozoa in clinical settings.
The findings suggest a need for primer redesign due to fungal dominance in sequencing, impacting routine clinical diagnostics.

Wang et al. (2025) studied this question.

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