Isolation of Primary Human Saphenous Vein Endothelial Cells, Human Internal Thoracic Artery Endothelial Cells, and Human Adipose Tissue-Derived Microvascular Endothelial Cells from Patients Undergoing Coronary Artery Bypass Graft Surgery
This approach synchronously collects human saphenous vein and artery endothelial cells, indicating potential for studying endothelial responses effectively.
Key Points
High efficiency in cell isolation (≈60% for HSaVEC, ≈50% for HITAEC) was achieved by using collagenase type IV.
The isolated endothelial cells displayed ≥99% purity, supporting their use in investigating endothelial heterogeneity.
Real-time bioimpedance monitoring demonstrated high proliferative activity of isolated endothelial cells, affirming their viability.
In vitro tube formation assays confirmed the angiogenic potential of human microvascular endothelial cells from various sources.