September 17, 2025ACS SensorsOpen Access

Structural and Kinetic Profiling of Rolling Circle Amplification via Solid-State Nanopore Sensing Using miR-21 as a Model

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This study combines rolling circle amplification with solid-state nanopore sensing, revealing kinetic changes in miR-21 analysis.

Time-dependent increases in translocation dwell time and current blockage suggest amplification kinetics evolve as RCA progresses.
The study utilizes RCA with solid-state nanopore sensing to analyze miR-21 at the single-molecule level, enhancing diagnostics.
Urea-PAGE and atomic force microscopy confirmed RCA results, while secondary structure predictions highlighted newly formed DNA configurations.
The developed signal extraction application improves reproducibility and provides clarity in event classification during sensing.

Loha et al. (2025) studied this question.

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