Review summarizes CRISPR/Cas systems for sensitive and multiplex detection of tumor biomarkers, highlighting their advantages and challenges.
Key Points
CRISPR/Cas-based detection strategies provide high sensitivity and specificity for tumor biomarker detection, overcoming limitations of traditional methods.
Recent advances in CRISPR/Cas systems enable multiplex target detection, which allows for the identification of multiple tumor biomarkers simultaneously.
Adoption of CRISPR/Cas systems in diagnostics improves operability and reduces cumbersome procedures compared to conventional methods like PCR and ELISA.
This review discusses challenges and future prospects of CRISPR/Cas approaches in tumor biomarker detection, underlining their transformative potential.