Enhanced Energized Dispersive–Guided Extraction Using Doehlert Matrix Optimization and Antioxidant Evaluation of Phenolic Compounds From Herbal Leaves by Using High‐Performance Liquid Chromatography
This research optimizes extraction using Doehlert matrix and evaluates antioxidant capacity in hydroethanolic extracts, suggesting synergistic effects among phenolic compounds.
Key Points
The optimized method significantly improved the yield of phenolic compounds and antioxidant properties.
Optimal conditions included 40% ethanol and 60% water at 165.82°C for 6.03 minutes, achieving a total phenolic content of 69.18 mg GAE/g.
High-performance liquid chromatography was used to reveal phenolic acids, including rutin and theophylline, enhancing the understanding of herbal constituents.
Correlation analysis indicated potential synergistic interactions among the active compounds found in the extracts.