September 12, 2025Acta ChromatographicaOpen Access

HPLC method development and validation for simultaneous analysis of methotrexate and leflunomide in bulk

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The developed HPLC method successfully quantifies methotrexate and leflunomide simultaneously, optimizing treatment strategies for arthritis.
Validation results show all tested parameters, including accuracy and precision, meet established criteria, ensuring method reliability.
A Shimadzu binary liquid gradient system is employed, detecting the drugs at 285 nm with elution times of 1.99 and 6.45 minutes respectively.
The method aligns with USP and ICH guidelines, highlighting its potential application in pharmaceutical quality control.

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Implication

Method development validates the simultaneous quantification of methotrexate and leflunomide, suggesting its effectiveness for rheumatoid arthritis treatment.

Cite This Study

Riaz et al. (2025) studied this question.

synapsesocial.com/papers/68d41c09713b0b5dfea659e2 https://doi.org/10.1556/1326.2025.01352

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Implication

Method development validates the simultaneous quantification of methotrexate and leflunomide, suggesting its effectiveness for rheumatoid arthritis treatment.

HPLC method development and validation for simultaneous analysis of methotrexate and leflunomide in bulk

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Discussion

Implication

Cite This Study

HPLC method development and validation for simultaneous analysis of methotrexate and leflunomide in bulk

Key Points

Discussion

Implication

Cite This Study