The enzymatic assay identifies compounds inhibiting ZIKV NS2B-NS3 protease, suggesting effective approaches against Zika virus.
This protocol describes the enzymatic assay used to identify compounds that inhibit enzymatic activity of the dengue virus protease (ZIKV NS2B-NS3 protease). Enzymatic assay is based on the conversion of substrate into product, which is used for quantification of enzyme activity. In case of using fluorogenic substrates, cleavage by the enzyme releases a fluorescent fragment, enabling the direct quantification of enzyme activity via fluorescence detection.
No takes yet. Share an insight, caveat, or question.
Zhadovets et al. (2024) studied this question.