Development of a LAMP‐Based Molecular Species Diagnostic Tool for the Invasive Pest, Tuta absoluta and Phthorimaea operculella (Lepidoptera, Gelechiidae)

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The assay achieved high sensitivity, detecting as little as 100 femtograms of genomic DNA.
Species-specific primers were optimized for use at 61°C to facilitate rapid detection in the field.
A simplified tissue-based DNA extraction method was established to support application in low-resource settings.
This tool aids in early detection efforts, significantly reducing economic losses in tomato production.

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LAMP assay reliably detects Tuta absoluta in tomato crops, highlighting its ease of use and specificity.

Hong et al. (2025) studied this question.

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