Data on the production of antioxidant exopolysaccharides produced by Bacillus amyloliquefaciens KW8 using cassava pulp and nitrogen supplement optimized via Response Surface Methodology

This study aimed to evaluate the optimal nitrogen source for the production of antioxidant exopolysaccharides (EPSs) by Bacillus amyloliquefaciens KW8 using cassava pulp carbon source. Various nitrogen sources were initially screened, and yeast extract was identified as the most effective. Further optimization was conducted using Response Surface Methodology (RSM) with a Central Composite Design (CCD) to assess the influence of key parameters, including pH, inoculum size, carbon-to-nitrogen (C/N) ratio, and agitation speed on EPS production. The amount of EPS, media pH, reducing sugar level, and viable bacterial population after 3 days of fermentation were recorded. The EPS's structural characteristics were investigated using scanning electron microscopy (SEM). The levels of bioactive compounds and antioxidant capabilities in the EPS, such as its total phenolic content, total flavonoid content, ferric reducing antioxidant power, hydroxyl radical scavenging activity, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were also measured. This experiment used microbial cell factories to convert cassava pulp, an agro-biowaste, into more valuable EPS bioproducts, which supports sustainable developmental goals.