September 10, 2025BIO-PROTOCOLOpen Access

An Optimized Enzyme-Coupled Spectrophotometric Method for Measuring Pyruvate Kinase Kinetics

View Full Paper

Ask AI

Bookmark

Authors

Discussion

This enzyme-coupled method measures pyruvate kinase activity in E. coli, suggesting its role in cancer metabolism.

The optimized method accurately measures pyruvate kinase activity, revealing its regulatory dynamics under varying conditions.
Key evidence shows that the LDH-coupled assay tracks NADH oxidation at 340 nm, enabling sensitive kinetic analysis.
Using a robust protocol, PKM2 was expressed in E. coli and purified to ensure high quality for enzymatic characterization.
This high-resolution method provides essential insights into PKM2 regulation, potential therapeutic targets, and cellular energy dynamics.

Saurabh Upadhyay (2025) studied this question.

View Full Paper

Ask AI

Bookmark