Simultaneous Quantification of Doravirine, Lamivudine, and Tenofovir Disoproxil Fumarate in Human Plasma by UPLC-MS/MS: Method Development and Validation

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This method validates the efficient quantification of DOR, LAM, and TDF in plasma, using ETR as an internal standard.

The developed UPLC-MS/MS method achieves high precision and reproducibility for drug quantification.
Mean recovery rates for Doravirine, Lamivudine, and Tenofovir were 83.39%, 87.33%, and 85.56%, respectively.
Utilizing Etravirine as an internal standard, the method demonstrated reliability across a concentration range of 2.5-1000 ng/mL.
Validated according to ICH M10 guidelines, this method ensures minimal degradation under stress conditions.

Kanjarla et al. (2025) studied this question.

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