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September 10, 2025Asian Journal of Microbiology Biotechnology and Environmental Sciences

Inclusion of Bromothymol Blue Dye in Conventional Pikovskaya’s Medium for Rapid Screening of Phosphate Solubilizing Bacteria

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Authors

SSS.K. SawaleAPA. S. PawarNPN.V. Phirke

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Overview

Plate assay shows enhanced visualisation of halo zones in phosphate solubilizing bacteria, indicating potential acid production.

Key Points

  • The modified assay improved visualisation of halo zones, supporting efficient identification of phosphate solubilizing bacteria.
  • Significant correlation between phosphate release and halo zone formation was found with an R value of 0.91248.
  • All 9 isolates displayed clear halo zone formation in the modified medium, suggesting effective phosphate solubilization.
  • This qualitative analysis protocol may facilitate rapid screening for bacteria that solubilize phosphate in various studies.

Cite This Study

Sawale et al. (2025) studied this question.

synapsesocial.com/papers/68c23f1fb210217d647951c5https://doi.org/10.53550/ajmbes.2025.v27i03-04.033
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