MicroRNA biomarkers in human embryo-endometrial implantation

Embryo-endometrial implantation is a crucial process in establishing pregnancy, with microRNAs (miRNAs) playing a significant role in regulating gene expression related to this process. Identifying miRNAs as biomarkers can provide valuable insights into predicting implantation success and improving outcomes in assisted reproductive technologies (ART). This study aimed to identify and validate significant miRNAs as biomarkers associated with human embryo-endometrial implantation, providing a comprehensive analysis of their potential in predicting reproductive outcomes. A systematic review and meta-analysis were conducted, including data from 50 studies that investigated miRNAs related to embryo-endometrial implantation. The frequency of miRNA identification, correlation with implantation success and robustness of findings were analyzed using bar charts, forest plots and sensitivity analyses. The study identified miR-451 and miR-21 as the most frequently reported miRNAs, with strong associations with implantation success. miR-30b and miR-30d were highlighted as enhancers of endometrial receptivity, while the miR-200 family was linked to the regulation of epithelial-mesenchymal transition (EMT). The findings were validated using independent datasets and sensitivity analyses confirmed the robustness of the results. miR-451, miR-21, miR-30b, miR-30d and the miR-200 family are critical biomarkers for embryo-endometrial implantation. Their identification provides valuable tools for predicting reproductive outcomes and offers potential for therapeutic interventions in ART. Further research is needed to validate these findings in diverse populations and explore their therapeutic potential.